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R287 PCN - DNA-based Detection Systems

Publication Date: 
18 August 2011
Author/Contact :
Author/Contact: 
Neil Boonham

Contractor :
Contractor: 
FERA, C&FR

Full Research Project Title: Validation of quantitative DNA detection systems for PCN
Duration: December 2006 - November 2008

Aim: To develop rapid, DNA-based diagnostics to quantify the amount of Globodera pallida and/or G. rostochiensis in a soil sample.

Current methods to detect and identify the potato cyst nematodes, Globodera pallida and G. rostochiensis are time consuming and require very highly skilled staff. However, recent developments in DNA-based detection systems for PCN suggest that a more rapid and reliable method of detection should be possible.

Researchers  at FERA (formerly Central Science Laboratory) and Crop and Food Research (New Zealand) worked together on this project to develop a quicker and more reliable system for the identification of PCN in soil samples.

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