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R294 Skin Spot Diagnostics

Publication Date: 
18 August 2011
Author/Contact :
Author/Contact: 
Jeff Peters

Contractor :
Contractor: 
FERA & SBCSR

Full Research Project Title: Determining the ability of a novel quantitative PCR assay to detect latent infections of Polyscytalum pustulans and its use as an early indicator of high risk seed
Duration: June 2007 - June 2008

Aim: To determine if DNA-based diagnostics can be developed for Polyscytalum pustulans and can be used to identify stocks with a high risk of developing skin spot.

Although the skin spot pathogen may be present on tubers at harvest, symptoms of the disease don't develop until many weeks later by which time controlling the disease is difficult. The aim of this project is to develop a test that will allow reliable, early detection of the skin spot pathogen - providing more opportunity to minimise disease development.

During the first phase of the project a PCR-based diagnostic assay for skin spot was developed. The assay was tested using tubers in storage and could successfully discriminate between low and moderate levels of skin spot based on the amount of pathogen DNA detected in tuber peel. The next phase of the project, which began in June 2007, will test the assay using tubers at harvest time.

The report on the initial phase of the project is provided below.

Reports and Key Words

 

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