Characterisation of Potato Cyst Nematode (PCN) Populations in Great Britain for Sustainable Crop Management
A stratified survey was conducted in England and Wales based on the total ware potato growing area. The aim was to collect 500 soil samples comparable to that reported in a previous survey (Minnis et al, 2002). The samples were systematically distributed across 34 counties. Samples were taken from 1 ha blocks
- Potato cyst nematodes were detected in 48% of samples obtained from ware potato growing land in England and Wales.
- Identification of PCN species from positive survey samples collected in England and Wales showed that 6% populations contained both species (mixed), 89% populations were pure G. pallida and 5% populations were pure G. rostochiensis.
Samples of 9 G. pallida field populations from the survey were selected for pathotype determination virulence tests. A range of potato genotypes, including a susceptible control and genotypes representing the main sources of resistance towards G. pallida pathotypes Pa1 and partial resistance towards Pa2/3, were used. 8 of the 9 samples could be assigned to pathotype Pa2/3. The remaining sample had high Pf/Pi ratios across all genotypes and could not be designated to G. pallida pathotype Pa1 or Pa2/3.
Determining the viability of eggs within the cysts is essential for assessing the effectiveness of control treatments and making decisions on inputs. The student assessed various techniques for estimating viability under identical conditions to identify the most reliable assay. Comparison of the assays showed that hatching in Potato Root Diffusate and a trehalose quantification assay mostly correctly detected samples containing non-viable nematodes.
Mitotype diversity assessment was carried out for a sub-set of the field samples.
Downloads115R471_Final Report Mitotyping report 2020
About this project
Aim: To characterise PCN populations of Great Britain by examining the distribution of species, virulence and viability.